Aptamer-based detection of fumonisin B1: A critical review

Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become relevant compound because of its adverse effects in food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD ELISA, while novel approaches integrate different sensing platforms fluorescently labelled agents combination with antibodies. Nevertheless, such could be expensive, time-consuming require experience. Aptamers (ssDNA) are promising alternatives overcome some drawbacks conventional methods, their high affinity through specific aptamer-target binding been exploited various designs attaining favorable limits detection (LOD). So far, two aptamers have reported, modified shortened sequences explored for successful target quantification. In this critical review spanning last eight years, we conducted systematic comparison based on principal component analysis aptamer-based techniques FB1, compared chromatographic, immunological other methods. We also an in-silico prediction folded structure both under reported conditions. The potential aptasensors future development highly sensitive testing emphasized. • An insight into health significance occurrence foods. A PCA new FB1. Recent advances immunosensors detection. Comprehensive determination aptamers.